PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN JENGKOL (Pithecellobium lobatum Benth) TERHADAP KADAR GLUKOSA DARAH PADA TIKUS PUTIH JANTAN (Rattus novergicus)

Gunawan Firmansyah; Hasty Martha Wijaya; Dian Arsanti Palupi; Nur Aini Karima; Bagus Riyanto

doi:10.31596/cjp.v9i1.331

PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN JENGKOL (Pithecellobium lobatum Benth) TERHADAP KADAR GLUKOSA DARAH PADA TIKUS PUTIH JANTAN (Rattus novergicus)

DOI: https://doi.org/10.31596/cjp.v9i1.331

Gunawan Firmansyah⁽¹⁾, Hasty Martha Wijaya^(2*), Dian Arsanti Palupi⁽³⁾, Nur Aini Karima⁽⁴⁾, Bagus Riyanto⁽⁵⁾

(1) Institut Teknologi Kesehatan Cendekia Utama Kudus
(2) Institut Teknologi Kesehatan Cendekia Utama Kudus
(3) Institut Teknologi Kesehatan Cendekia Utama Kudus
(4) Institut Teknologi Kesehatan Cendekia Utama Kudus
(5) Institut Teknologi Kesehatan Cendekia Utama Kudus
(*) Corresponding Author

Abstract

Diabetes mellitus is a type of chronic disease with specific characteristics with blood glucose levels above normal. Jengkol leaves are one part of the plant that contains flavonoids, saponins and tannins that can be used as a treatment for DM. This study aims to understand the impact of administering ethanol extract of jengkol leaves (Phitecellobium lobatum Benth) on blood glucose levels in male white rats (Rattus novergicus). This study is an experimental study applying a pretest and posttest control group design. After checking glucose levels on H0, the rats were induced using alloxan monohydrate intraperitoneally. The rats were separated into 5 groups including the positive control group given metformin, the negative control group given Na-CMC, control groups 1, 2, 3 were given ethanol extract of jengkol leaves with a continuous dose of 500, 1000, 1500 mg, given orally for 14 days. The checks were carried out on H7 and H14 after being given alloxan induction. The results of the study were analyzed using the Shapiro Wilk normality test and data homogeneity test. Then continued One Way ANOVA analysis and Tukey Post Hoc test on H7 and H14 treatments. The results showed no significant difference in the positive control with the 1000 mg and 1500 mg dose groups, this indicates that both doses of jengkol leaf ethanol extract have a balanced ability with the positive control group. Ethanol extract of jengkol leaves has an effect on reducing blood glucose levels in male Wistar strain rats and the effective dose of jengkol leaf ethanol extract on blood glucose levels in male white Wistar strain rats given alloxan monohydrate is 1000 mg/KgBW.

Keywords

Jengkol leaves; glucose levels; alloxan

Full Text:

PDF

Article Metrics

Abstract viewed : 4 times |

PDF files downloaded : 2 times

References

Depkes, R. I. (2000). Parameter standar umum ekstrak tumbuhan obat.Jakarta: Departemen Kesehatan Republik Indonesia.

Djuwarno, E., & Abdulkadir, W. (2019). Penurunan Kadar Glukosa Mencit Akibat Pemberian Kombinasi Metrformin dan Ekstrak Bawang Merah. Journal Syifa Sciences and Clinical Research (JSSCR), 1(1): 8–13.

Husni, E., Suharti, N., & Atma, A. P. T. (2018). Karakterisasi simplisia dan ekstrak daun pacar kuku (Lawsonia inermis Linn) serta penentuan kadar fenolat total dan uji aktivitas antioksidan. Jurnal Sains Farmasi & Klinis, 5(1): 12–16.

Kemenkes, R. I. (2019). Pedoman pelayanan kefarmasian pada diabetes.Jakarta: Kementrian Kesehatan Republik Indonesia.

Kurniawan, D. A. (2020). Flavonoid pada buah jengkol (Pithecellobium lobatum Benth) sebagai terapi alternatif diabetes melitus tipe 2. Wellness And Healthy Magazine, 2(2): 375–382.

Lolok, N., Yuliastri, W. O., & Abdillah, F. A. (2020). Efek antidiabetes kombinasi ekstrak etanol daun pandan wangi (Pandanus amaryllifolius Roxb.) dan daun salam (Syzygium polyanthum Wight.) pada tikus putih dengan metode induksi aloksan. Jurnal Mandala Pharmacon Indonesia, 6(01): 13–29.

Malini, D. M., Madihah, M., Kusmoro, J., Kamilawati, F., & Iskandar, J. (2017). Ethnobotanical study of medicinal plants in Karangwangi, District of Cianjur, West Java. Biosaintifika: Journal of Biology & Biology Education, 9(2): 345–356.

Megawati, A., & Pujiastuti, E. (2018). Pengaruh Ekstrak etanol ranting buah parijoto (Medinilla speciosa Blume) terhadap kadar glukosa darah tikus putih dengan metode induksi aloksan.Cendekia Journal of Pharmacy, 2(2): 95– 101.

Putra, I., Dharmayudha, A., & Sudimartini, L. M. (2016). Identifikasi senyawa kimia ekstrak etanol daun kelor (Moringa oleifera L) di Bali. Indonesia Medicus Veterinus, 5(5): 464–473.

Sugiarti, L., & Fitrianingsih, S. (2018). Aktivitas antibakteri ekstrak etanol daun parijoto (Medinilla speciosa Blume) terhadap pertumbuhan bakteri propionibacterium acnes dan staphylococcus aureus. Cendekia Journal of 12 Pharmacy, 2(1): 60–67.

Sukandar, D., Zahroh, H., & Amelia, E. R. (2012). Uji aktivitas antidiabetes fraksi etil asetat daun pandan wangi (P. amaryllifolius Roxb.) dengan metode Î ‘- Glukosidase. Jurnal Riset Sains Dan Kimia Terapan, 2(1): 124–129.

Syafnir, L., Krishnamur, Y., & Ilma, M. (2014). Uji aktivitas antidiabetes ekstrak etanol kulit jengkol (Archidendron pauciflorum (BENTH.) IC NIELSEN. Prosiding SNaPP: Sains, Teknologi, 4(1): 65–72.

Wahyuni, R., & Guswandi, H. R. (2014). Pengaruh Cara Pengeringan Oven, Kering Oven, dan Cahaya Matahari Langsung terhadap Mutu Simplisia Herba Sambiloto. J. Farmasi Higea, 6(2): 132.

Yulia W. R., Susilo, J., & Dyahariesti, N. (2020). Perbandingan aktivitas antioksidan ekstrak etanol terpurifikasi ubi jalar ungu (Ipomoea batatas. L) dengan vitamin e. Disertasi telah diterbitkan. Semarang: Universitas Ngudi Walyo.

Yunitasari, D., Alifiar, I., & Priatna, M. (2016). Uji aktivitas ekstrak etanol daun jengkol (Pithecellobium lobatum benth) terhadap penyembuhan luka insisi pada tikus putih jantan strain Wistar. Jurnal Farmasi Sains Dan Praktis, 2(1): 30–35

DOI: https://doi.org/10.31596/cjp.v9i1.331